FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082119
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.1632907286536878No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.15118485120398034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48850.039.9559251
TGGTATC14100.039.1828842
GTGGTAT14500.037.8316081
TATCAAC56100.035.07442
GGTATCA25350.033.8849261
ATCAACG58750.033.4923253
TCAACGC58750.033.4923254
AACGCAG59250.033.353146
CAACGCA60300.032.8428425
ACGCAGA65350.030.2398437
CGCAGAG66900.029.6027438
GTCGAGG6200.028.7892118
GCAGAGT73450.027.020749
AGCGTCA2750.023.1809233
TCGAGGT7300.022.7046039
CAGAGTA47450.022.61555910-11
GCGTCAG2951.8189894E-1221.6093354
AGAGTAC45400.021.39008710-11
CGTCAGA3051.8189894E-1220.9008335
GTACGGA1909.526575E-720.14291
Produced by FastQC (version 0.11.5)