FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969877
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16530.17043398286586856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19900.038.676092
GTGGTAT20500.037.8317871
GTATCAA65550.024.9215681
AGAGTAC46750.023.47094211
CGACCGC600.002030853622.9252950
CAGAGTA48300.022.65962810
TATCAAC72900.022.3219322
GGTATCA36150.022.1235643
TAGACGG752.8709153E-422.00146714
ATCAACG78400.020.752773
TCAACGC78100.020.7269154
AACGCAG78150.020.7125876
CGATCGT804.4303213E-420.63276347
CAACGCA79700.020.34535
AGTCCGG1108.233286E-620.00442954
GACAGGT3700.018.582321
GAGTACG58900.018.53593312
AGTACGG58950.018.38120813
ACGCAGA87950.018.3421527
GTCCGGA1051.3063378E-418.33360955
Produced by FastQC (version 0.11.5)