FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995843
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26870.2698216485932019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32350.030.0289021
AGAGTAC33650.026.88307211
TATCAAC36350.026.8556462
AACGCAG36150.026.691916
ATCAACG36650.026.3290843
CAGAGTA34350.026.17382810
TCAACGC36950.026.0396064
ACGCAGA37350.025.8343377
CAACGCA37950.025.5707655
CGCAGAG38050.025.2880888
GTGGTAT7900.024.7326281
GGTATCA15300.024.1019611
GCAGAGT40150.024.0351039
TGGTATC8050.023.5702882
AGTACGG39250.022.28029613
GAGTACG40250.022.13446212
TACGGGC7050.018.33520915
TATACCG750.007426171618.3269215
GTACGGG47100.018.1007314
TACGGGG18700.018.09008615
Produced by FastQC (version 0.11.5)