FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769466
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.44225475849485224No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.4179521902202306No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.2505633777190935No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.23171914028690027No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.17817551392784087No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12800.16634913043591268No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11900.15465270720213758No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9360.12164280163126115No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA9050.1176140336285164No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8840.1148848682073022No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT8020.10422812703875155No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA7920.10292852445722098No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG7760.10084916032677207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG1850.048.261841
TGGTATC27350.037.138772
GACAGGT2600.034.3401531
GTGGTAT29100.034.188431
GTATCAA79800.033.192611
CAGGTAT4750.032.2229421
AACGCAG86200.031.2585586
GCTTGTA4700.029.8519291
TATCAAC91550.029.4782922
TCAACGC95250.028.3778214
GCTTATA1652.7830538E-1028.3442541
GACGCTA600.0068043628.3332049
ATCAACG96700.028.0402013
CAACGCA97100.027.8371525
ACGCAGA97050.027.8077057
CGCAGAG98050.027.4807518
GTCGAGG11250.026.444328
GGTATCA49350.026.2765881
TTGTACG1308.0228165E-726.1537253
GCAGAGT105200.025.69389
Produced by FastQC (version 0.11.5)