FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769466
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA11320.1471150122292603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10880.1413967608705258No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8730.11345530536761858No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8340.10838685529964937No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7820.10162892187569042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG704.090907E-935.348413
CGATCGT558.68964E-735.0027347
AGTCCGG658.381721E-833.84879354
GTCCGGA601.7178027E-632.0795855
GACAGGT2000.027.5164571
CGGACTA507.029257E-427.5003579
TCCGGAC400.00787853627.4949957
TGGTATC29250.027.449782
GCTTGTA3150.027.0796851
GTGGTAT30650.026.2146991
CCGATCG759.74598E-625.66746
CGGGACC1101.19289325E-824.9938244
CCTATGC550.00122795324.9938243
GTATCAA92050.024.7812521
TAGTCCG901.4450434E-624.44635253
AGAGTAC61300.023.69017411
CAGAGTA61500.023.61313410
CGGGATA1750.023.574817
AGACAGG1900.023.1717551
AACGCAG99250.022.6593886
Produced by FastQC (version 0.11.5)