FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347138
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.21374784667769015No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.18897383749402255No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.12502232541525274No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.11033076182958938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21450.033.095311
AACGCAG22950.032.034326
GTCGAGG3150.031.0291188
ACTCTAC703.9329333E-430.3633171
TATCAAC24600.029.7129292
TCAACGC25600.028.5522694
ATCAACG25850.028.2761333
ACGCAGA26600.027.9581577
CGCAGAG26800.027.7495168
CAACGCA26750.027.3247875
ACGGGAC4050.027.281643
CGGGACC957.4394855E-526.839834
GCTTGTA1457.021663E-826.3846781
GCAGAGT28500.026.2433919
GTGGTAT8050.025.8748281
TGGTATC8450.024.6428812
GGTATCA13300.023.971041
TCGAGGT3800.023.4848529
CAGAGTA17900.023.26618610-11
CAGGTAT1852.9021976E-822.9776461
Produced by FastQC (version 0.11.5)