FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347138
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4420.12732688440908227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGC257.8374206E-444.0130452
TATACGG300.001930517736.6405375
GCTTGTA1100.035.005321
CGGCTGT350.004089786731.43788347
ACCGACT400.00785431327.5081550
CGATCGT507.0097984E-427.50814847
GTCTAAT705.6851495E-627.504181
TCTGGCG400.00788199827.48832531
TTGTACG507.0512807E-427.4804063
CCGATCG550.001223741925.00380146
AGAGTAC18050.024.98274211
CAGAGTA18450.024.44111310
GTATCAA25000.023.6535951
ATCGTAG701.793488E-423.57841349
AACGCAG26400.022.3798736
TGGTATC9350.022.0494582
ATTAGGC752.8809212E-421.9843223
GTGGTAT9100.021.761551
TATCAAC27500.021.2909562
GTCCGGA650.00323780621.15096355
Produced by FastQC (version 0.11.5)