FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063801
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37640.35382557452004654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7200.036.3090631
TGGTATC7800.032.079192
GTATCAA36600.028.4206941
GGTATCA17900.025.6736371
TATCAAC41550.024.8162922
ATCAACG43100.024.0423743
TCAACGC43500.023.6938084
TATACCG701.8078291E-423.5573085
CAACGCA44450.023.3099885
AGAGTAC41450.023.01657511
AACGCAG45550.022.7502756
CAGAGTA42850.022.5191110
ACGCAGA46450.022.4278267
CGCAGAG46700.022.249958
TGCCGAC4350.021.50057855
GCCGACG1701.2551027E-1021.03559955
GCAGAGT50000.020.7274519
TTATACG804.4359788E-420.62912835
AGTACGG45900.020.25078613
CGCCGTA700.004982220519.63847425
Produced by FastQC (version 0.11.5)