FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046104
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15610.14922034520468327No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC12940.12369707027217178No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT12050.11518931196133464No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG11670.11155678594097719No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGCG150.00577787755.01344352
TGGTATC21150.030.8010332
GTGGTAT21950.029.9389131
CGGAGCT507.027346E-427.50277517
CGCGAGC1156.657501E-1026.29819531
GTATCAA71000.025.7922081
GCTTGTA3850.024.9965441
AGCCGGT550.001228154424.99415231
CCTTCGC1351.6007107E-1024.44106520
AGTCCGG801.6067957E-524.06492654
AGAGTAC56400.023.60167111
CAGAGTA56700.023.52530110
GACAGGT3050.023.4393831
GTCCGGA852.5708807E-522.64609355
TATCAAC82550.022.209362
AACGCAG83050.022.0094556
ATCGGTA1505.966285E-1022.00327128
CTTCGCC1505.984475E-1022.00011421
GGCCGAC752.8732466E-421.99906255
AGCCGAC1801.0913936E-1121.39104741
Produced by FastQC (version 0.11.5)