FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2287793
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.17108191169393386No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.1643505334617249No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.11041208710753114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC27050.043.2049032
GTGGTAT27450.043.0642551
GTATCAA95550.038.8506281
GGTATCA53650.035.745491
TATCAAC110650.033.7602042
TCAACGC112700.033.146114
ATCAACG115100.032.5288123
CAACGCA115100.032.4549645
AACGCAG115350.032.4214676
ACGCAGA127550.029.2877167
CGCAGAG129350.028.8473018
GCAGAGT141100.026.5354219
GTCGAGG12750.024.999328
CAGAGTA87600.023.91825110-11
GACAGGT13150.021.0185171
AGAGTAC84800.020.0721210-11
GAGTACG109100.018.91300812-13
TACGGGG46550.018.624222
AGTCGAG21650.018.4521527
TCGAGGT16750.018.0144359
Produced by FastQC (version 0.11.5)