FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761088
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44070.2502430315804775No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43090.24467828978449688No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27970.15882227350365227No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.12957898753497837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC29550.047.8921052
GTGGTAT29550.047.042541
GTATCAA91800.035.564691
GGTATCA53200.032.2827261
TATCAAC103800.031.9355532
AACGCAG109200.030.395246
ATCAACG109900.030.12433
TCAACGC110250.030.105764
CAACGCA112650.029.6152675
AGCGTCA3800.029.078163
ACGCAGA123450.026.852257
CGCAGAG126600.026.1505578
GTCGAGG14500.024.9131188
GCAGAGT135850.024.4012559
TCGAGGT15550.022.1376639
CAGAGTA79050.021.72015210-11
GCGTCAG5150.021.4557294
TCCGGAC1100.00548134319.317663
AGAGTAC74200.019.30231310-11
ACGGGTC5100.019.1661475
Produced by FastQC (version 0.11.5)