FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814959
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.3332682993868403No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23310.28602665900983976No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.20724968986169856No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13460.1651616829803708No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.15043701584987712No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT11660.14307468228463027No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC11340.1391481043831653No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.12933165962950285No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG10440.12810460403529503No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.12025144823236507No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA9250.11350264246422212No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8890.10908524232507402No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA8640.10601760333955451No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC8490.1041770199482428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13600.048.4345632
GTGGTAT13850.047.2737581
GTATCAA53350.040.7226141
TATCAAC59900.036.2540632
GGTATCA27150.035.0773961
ATCAACG64100.033.87863
TCAACGC64550.033.510754
AACGCAG65350.033.230586
CAACGCA65800.032.9387325
ACGCAGA72800.029.771557
CGCAGAG73200.029.5508028
GCAGAGT77650.027.9667539
GTCGAGG9200.025.8679968
CGTCAGA1401.5313981E-624.2842455
CAGAGTA43550.023.81099710-11
GACAGGT3600.021.2578411
AGTCGAG13550.021.0134857
AGAGTAC41900.020.9957910-11
TCGAGGT11150.020.5817139
GAGTACG50300.020.40508312-13
Produced by FastQC (version 0.11.5)