FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814959
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18410.2259009348936572No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT12420.1524003048006096No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT10450.1282273095947158No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC10220.12540508172803785No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC9130.11203017575117276No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA8200.10061855872504015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18400.039.2895662
GTGGTAT18800.038.6140481
CTCTCGA507.033314E-427.4978831
GTATCAA66650.026.1982821
AGAGTAC44100.024.8836211
ACGGGCC1551.8189894E-1224.84137216
CAGAGTA45300.024.4050910
TATCAAC73500.023.7105272
TTCCGCG701.7979403E-423.57540146
AACGCAG77200.022.5699866
TCAACGC77300.022.4341284
ATCAACG77450.022.4261653
CAACGCA78300.022.288015
GGTATCA34800.021.8086641
CATTCGT903.9923783E-521.38986810
CGGGACC903.9923783E-521.38986817
AGTACGG50600.021.36098313
CGAGGTT9750.021.15481610
GAGTACG51750.021.15202512
GACAGGT2750.020.9983831
Produced by FastQC (version 0.11.5)