FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050103
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT48270.4596691943552204No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46590.44367076372508224No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.33463384068039037No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24130.22978698280073478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14250.056.079131
TGGTATC14150.055.8640062
GTATCAA58000.040.3814471
TATCAAC63600.036.6851922
GGTATCA29750.036.2915081
TCAACGC66900.034.812084
ATCAACG67950.034.336693
CAACGCA68050.034.1613275
AACGCAG71200.032.7096676
CGCAGAG75250.030.9462648
ACGCAGA75500.030.8437947
GCAGAGT79050.029.4586529
CGTCAGA2053.6379788E-1226.9503885
CAGAGTA51150.026.08664310-11
GAGTACG56550.023.63374512-13
CTATCGG1152.7205207E-422.1774541
AGAGTAC50200.022.00917610-11
GTACGGG61900.021.62592914-15
ACGGGAT13050.020.67845316-17
CCTCACG1455.5061864E-520.516614
Produced by FastQC (version 0.11.5)