FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050103
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96390.9179099574041785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10850.035.9966161
TGGTATC11450.033.612282
GTATCAA51500.028.6259961
GGTATCA24650.026.8907661
TATCAAC57700.025.3937512
TCAACGC60300.024.2953594
AGAGTAC54250.024.07765211
CAACGCA61300.023.9461425
ATCAACG61900.023.9315133
CAGAGTA54800.023.8325910
AACGCAG63100.023.3959716
GGGACGC600.00203349622.9203838
ACGCAGA65300.022.4814457
CGCAGAG65100.022.4681938
GCAGAGT68300.021.3762749
AGTACGG60100.021.32523513
GAGTACG60900.021.26786412
ACGGGGC4400.021.25242816
TACGGGG23600.020.16120515
GTATACC700.004971091619.646041
Produced by FastQC (version 0.11.5)