FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496375
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.2484003007267563No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.23082449252359868No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.15711302313925318No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT21360.14274496700359202No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.10264806615988639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25100.045.206572
GTGGTAT25850.044.2444651
GTATCAA77600.037.257561
TATCAAC87900.033.50492
TCAACGC93450.031.4695684
ATCAACG93400.031.4409163
AACGCAG94850.031.1842926
CAACGCA95550.030.8668845
GGTATCA44100.029.3090821
ACGCAGA108150.027.3493337
CGCAGAG108600.027.2751378
GTCGAGG14000.026.4091998
GCAGAGT115850.025.6416029
TCGAGGT16150.022.3671389
CAGAGTA68150.021.67006510-11
AGTCGAG20950.020.893797
AGAGTAC65150.019.89558210-11
AGTACGG84100.019.505422
AGCGTCA3359.094947E-1219.0287593
TTGTACG1801.3524836E-518.8878043
Produced by FastQC (version 0.11.5)