FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496375
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20060.13405730515412248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28200.035.98031
TGGTATC28950.034.943842
GTATCAA96350.024.4006791
TTGTACG801.6141199E-524.0519583
AGAGTAC69150.022.5907611
TATCAAC105900.022.3240972
CAGAGTA70700.022.01768710
TCGAGCG752.8726397E-422.00065618
GGTATCA53450.021.6581781
ATCAACG114850.020.5351873
AACGCAG114800.020.4004656
TCAACGC115150.020.38624
GCTTGTA4250.020.0567381
CAACGCA117250.020.0210745
CTAGCGG700.00497105519.64672749
ACGCAGA127900.018.3330767
CGCAGAG129800.018.0453518
AGTACGG85550.017.87431713
GAGTACG86800.017.77533512
GTATTAG4500.017.720381
Produced by FastQC (version 0.11.5)