FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707730
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40320.5697087872493747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6350.038.536672
GTGGTAT6600.037.9396321
GTATCAA29300.029.958411
TATCAAC33400.026.6720522
AGAGTAC32900.025.65667511
CAGAGTA33650.025.16476410
ATCAACG35300.024.9957083
GGTATCA12950.024.6481271
TCAACGC35700.024.6386494
CAACGCA36300.024.5342925
AACGCAG37550.023.8622886
CGCAGAG38150.023.2741478
ACGCAGA39150.022.9589027
CCGCTGT600.002032617622.9208248
ACGGGTC1354.2418833E-922.408316
AGTACGG36850.022.3889513
GCAGAGT39650.022.325919
ACGGGGC1850.022.29818516
TGCCGAC3100.022.18614655
GAGTACG37450.022.09899712
Produced by FastQC (version 0.11.5)