FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224515
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22370.18268457307587085No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT14620.11939420913586196No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12660.10338787193296937No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT12520.10224456213276277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT24500.038.4991721
TGGTATC24750.037.759992
GTATCAA90550.027.3627451
AGAGTAC60500.025.00457211
CGGGTCG550.001226169925.00150517
TATCAAC100700.024.3753092
CAGAGTA62900.024.2691410
TCAACGC107500.022.8287664
ATCAACG108750.022.6169073
AACGCAG109450.022.3996776
CAACGCA109950.022.3450725
GGTATCA45200.021.827073
AGTACGG69800.021.43658313
GAGTACG72100.020.94349512
ATAGCGG804.4554126E-420.616137
TACGGGG23500.019.89725315
ACTATCG1251.1214925E-619.8036211
CGTACGA700.004974482619.6440435
ACGCAGA125400.019.5749117
ACGGGAT9450.019.49932516
Produced by FastQC (version 0.11.5)