FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145502
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.10631544505668136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13500.034.9490551
GGTATCA24900.034.141051
GTATCAA42900.032.399381
TGGTATC13950.032.295162
CAACGCA49400.029.3388615
TATCAAC52000.028.525142
ATCAACG51350.028.390273
TCAACGC52450.027.6327884
AACGCAG53000.027.3460356
GTCGAGG6150.026.2587288
ACGGGCG2650.025.6619685
CAGTGCG5200.025.3351559
GGCGTCG2550.024.9986368
ACGCAGA58700.024.6164997
GCGGCGA2100.024.2855230-31
TGCGCGT2450.024.28495412-13
GCGTCGG2451.8189894E-1224.2843889
CGCAGAG59300.024.2229638
CGTTACG2400.023.9063448-49
CGGGCGT2950.023.0522776
Produced by FastQC (version 0.11.5)