FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145502
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38230.17818673671709465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA300.001924980536.67059743
CGTAACG1550.031.93518623
GTGGTAT14150.031.8858781
ACGTTCG701.6084778E-731.43194244
ATCGCGA350.00409826731.43194242
TGGTATC14050.031.1185282
TCGCCGG852.7117494E-829.11737616
GTACGCA1800.029.0268311
CGGTCCG1251.8189894E-1228.59973328
GCGACGT1650.028.3343935
TCGCCGA400.00787050927.50294941
AATCGCG1303.6379788E-1227.50294941
TACGTAT904.7370122E-827.4920525
AGCGACC2200.026.24975617
CGCAGCG2000.026.12353914
CCGTAAC1900.026.05360422
ATCGGTC1500.025.66582726
CGCCTAC1500.025.6592467
TCGACTC550.001226134225.00268240
TCGCCGC1601.8189894E-1224.06507940
Produced by FastQC (version 0.11.5)