FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096020
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38810.18516044694230016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT32900.042.545131
TGGTATC33200.041.3974272
GTATCAA124750.027.6650311
AGAGTAC83150.025.43510411
TATCAAC137150.024.912472
CAGAGTA85750.024.7280410
GGTATCA61100.023.3928343
TCAACGC148000.022.9735814
ATCAACG148700.022.9024033
CAACGCA150200.022.6187845
AACGCAG153250.022.2055536
AGTACGG97750.021.52407613
ACGGGTC2700.021.3912116
GAGTACG98850.021.33969112
GACAGGT9100.021.1536181
CGCGGTA1108.250539E-620.00169212
CGCAGAG172750.019.6709238
ACGCAGA172750.019.6362827
ACGGGCT5050.019.60608916
TACGGGT10050.019.42996815
Produced by FastQC (version 0.11.5)