FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933751
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT94470.3220109682110036No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.3140689172325804No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60250.20536848560085705No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.17070296695254641No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.12403915669734752No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT32560.11098419736371627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC55450.047.364092
GTGGTAT59700.043.7269131
GTATCAA208150.040.279611
TATCAAC238850.035.5316282
AACGCAG243950.034.7191246
ATCAACG245050.034.6326453
TCAACGC245000.034.6223684
CAACGCA248500.034.1176265
GGTATCA108300.033.5654451
ACGCAGA268750.031.5479777
CGCAGAG271400.031.271798
GCAGAGT289700.029.370249
CAGAGTA179550.024.25140610-11
GGCACCG9650.023.3421279
GACAGGT14750.022.7714041
CGGGTCA7800.022.3383736
GTCGAGG26900.022.2767248
AGAGTAC175250.021.16010310-11
GGTACGG5700.020.8759123
GCTTGTA15050.020.6224921
Produced by FastQC (version 0.11.5)