FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456110
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59720.41013384977783274No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44870.30814979637527384No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.23741338223073807No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.1622130196207704No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22600.15520805433655424No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.11427708071505586No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.10150332049089698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19700.057.8148272
GTGGTAT20400.054.8063051
GTATCAA81550.041.025671
TATCAAC90850.037.2824172
GGTATCA40550.036.588071
ATCAACG99200.034.1870733
TCAACGC100100.033.7098734
CAACGCA102200.033.058795
AACGCAG105400.032.0551036
GTCGAGG16650.028.589358
ACGCAGA121550.027.7261077
CGCAGAG122200.027.5805238
TCGAGGT16950.026.5797949
GCAGAGT128650.026.2977549
AGTCGAG24250.024.35987
GACAGGT8050.024.29221
AGCGTCA2750.023.1808433
CAGAGTA61650.023.09548810-11
GCGTCAG2951.8189894E-1221.6092624
AGAGTAC59700.021.25129710-11
Produced by FastQC (version 0.11.5)