FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456110
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26470.18178571673843322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC26000.043.7569352
GTGGTAT26500.043.469231
GTATCAA104600.027.5450441
GACAGGT6150.025.927861
AGAGTAC66250.025.57423811
CAGAGTA67550.025.08206210
TATCAAC115800.024.917282
GGTATCA52450.023.6922761
ATCAACG123350.023.2584763
TCAACGC124400.023.0392824
CAACGCA126250.022.679135
AGTACGG75200.022.60364713
AACGCAG127650.022.4542376
GAGTACG77450.021.87596112
CGGGATC7300.020.72134217
GTACGGG84200.020.05691514
TACGGGG32600.019.82705115
ACGCAGA145550.019.7130097
TTCGCGT700.004971677419.6462610
ACGGGGA14100.019.50625216
Produced by FastQC (version 0.11.5)