FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946449
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.2603415503635167No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23710.2505153473668417No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.15257029169030767No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.1332348599871731No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA11960.1263670836991745No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC11270.11907667502422212No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC10910.11527298354163827No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA10500.11094100157536223No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.10882783964059342No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA9900.10460151577105581No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9780.10333361861019452No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT9490.10026953380477976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69150.036.8916051
AACGCAG73450.035.4115146
TCAACGC79900.032.659274
ATCAACG80450.032.541653
CAACGCA80600.032.4283565
TATCAAC80350.032.2118952
TGGTATC22050.031.9951692
AAGTACG1605.456968E-1231.8881821
ACGCAGA82200.031.6937457
CGCAGAG83250.031.2940048
GTGGTAT22900.030.4492661
ACAGGTA5200.030.252891
GCAGAGT89450.029.1724519
GACAGGT3300.028.345051
GGTATCA40600.026.4949441
CGGGGCA3700.026.4186734
CGGGCTC1051.4704504E-424.2854884
CAGAGTA56250.024.10199710-11
GTCGAGG8300.023.5539998
ACGGGTA4200.023.2735945
Produced by FastQC (version 0.11.5)