FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824483
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26190.31765360838246515No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.2661061537957726No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.18447924335613955No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.14845666920967443No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.12516934854933334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11750.056.062832
GTGGTAT12850.052.262221
GGTATCA23050.037.8021741
GTATCAA40850.035.793181
TATCAAC46650.031.6125142
ATCAACG49700.029.8435343
TCAACGC50100.029.605264
CAACGCA51150.029.1637025
AACGCAG52550.028.5484926
GTCGAGG8000.026.0307858
ACGCAGA60000.025.0037217
CGCAGAG59950.024.9536828
GCAGAGT63100.023.842689
TCGAGGT8100.023.610699
AGTCGAG12000.023.3745827
CAGAGTA33600.023.27480710-11
ACGGGCC950.002342791522.3680238
GACAGGT4200.022.264211
TAGACTA1455.5037985E-520.5168745
ATACGCC1254.782413E-420.3996376
Produced by FastQC (version 0.11.5)