FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824483
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20570.24948968019959175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12950.042.2390632
GTGGTAT13650.040.4830671
CTAGACT300.00193085336.645194
CGTTCTA553.486265E-530.00427226
GTATCAA52000.025.8004021
GGTATCA27050.025.1038131
TATCAAC57650.023.1722342
AGAGTAC36200.022.793311
CAGAGTA37200.022.32844710
TCAACGC61100.021.7712044
ATCAACG61700.021.6040343
CAACGCA62800.021.3131475
AACGCAG63450.021.2234696
CCGTTAC650.003236308221.15685814
TCGCGTA650.00323744821.15557341
CGCGTAC700.00497425719.64326942
CTCGCAC700.00499174519.6313533
AGTACGG41600.019.43786213
ACGCAGA71350.018.9166767
CGCAGAG71650.018.726928
Produced by FastQC (version 0.11.5)