FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846532
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.2959039280078355No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT79760.2802006090217851No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49660.1744579017555397No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.15422275245807882No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA41050.1442105692119393No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC37780.1327229063295266No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA36220.12724255339479762No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA33790.11870584978493127No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT33030.11603593425262741No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC32520.11424428040858138No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.11354167105797511No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC32070.11266340936971725No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT31920.11213645235676253No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.1115392344087472No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG29890.10500496744810879No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA28630.10057852853928922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC65000.037.9215472
GTATCAA218150.037.810841
AACGCAG239400.034.7761656
GTGGTAT69850.034.5132941
TATCAAC256050.032.7637672
TCAACGC255900.032.6335034
ATCAACG259700.032.188733
CAACGCA260750.032.1080065
ACGCAGA268200.031.0259697
CGCAGAG271100.030.694088
GCAGAGT290000.028.7532989
CTTGAGT8750.028.6559872
GGTATCA124400.027.855251
GCTTGTA16200.027.8202271
GTCGAGG26950.027.596298
GCTTGCC4500.026.4554621
GACAGGT11800.025.5826871
CAGAGTA174250.024.13423310-11
TTGTACG3900.023.9733923
AGACAGG12500.022.789491
Produced by FastQC (version 0.11.5)