FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846532
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43540.1529580556269875No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA38740.13609543121243675No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC35870.1260129870312366No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT32900.11557923817473333No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG32640.11466584601894515No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT32550.11434967181117232No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT30310.10648044708438198No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC29050.10205400817556241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC74100.031.713642
GTGGTAT76200.030.9565531
GTATCAA243350.025.6683081
AGAGTAC176950.024.24808911
CAGAGTA179050.024.19283710
AACGCAG267800.022.9692966
TATCAAC272600.022.7967472
TCAACGC279400.022.0334054
TTAAACG6250.022.00358622
ATCAACG281000.021.9568523
CAACGCA284150.021.6360685
TTGTACG2300.021.508233
GACAGGT9250.021.1026441
GCACCGC11500.020.80664310
ATTAGAG12450.020.5292763
ACGCAGA299950.020.5091447
GGCACCG11700.020.4484589
CGCAGAG302150.020.3168268
GCTTGTA12300.019.8932251
GCAGAGT318700.019.302399
Produced by FastQC (version 0.11.5)