FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182653
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77830.6580966690990511No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51600.4363071839330725No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.35623297788954156No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.2534978560913472No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT29430.2488472950222931No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.18686799932017253No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.1571889641340275No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17790.15042451166994883No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.14272994699205938No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.11245902221530746No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.10594823671863177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22450.059.8349531
TGGTATC25650.052.6882482
GTATCAA98450.038.2562331
GGTATCA52650.035.6868441
TATCAAC108550.035.2360082
GACAGGT5250.034.007461
GTCGAGG16950.033.8483968
TCAACGC116000.033.0096444
ATCAACG117150.032.6493263
CAACGCA119600.032.0515755
AACGCAG121500.031.5853376
TCGAGGT18650.029.8527859
ACGCAGA135700.028.1548927
CGCAGAG138100.027.7271448
GCAGAGT142150.026.8486149
CAGAGTA67950.024.86222810-11
CGGGTCA1401.5320238E-624.2848826
AGTCGAG28850.023.716697
TACGCCT2104.5838533E-922.2611397
GAGTACG77500.021.57920312-13
Produced by FastQC (version 0.11.5)