FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182653
Sequences flagged as poor quality0
Sequence length61
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28290.23920795026098102No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17050.1441673931406761No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.1431527252710643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT28300.042.8495671
TGGTATC29000.041.899452
GTATCAA117200.027.8260361
AGAGTAC70550.027.36462411
CAGAGTA70900.027.34590310
TATCAAC128300.025.4978982
AGTACGG80550.023.7284113
GAGTACG80950.023.7130812
GGTATCA56150.023.6532971
TCAACGC138400.023.3968054
GACAGGT4350.023.3887371
ATCAACG138950.023.3447463
ACGGGGC5100.023.18716616
CAACGCA141450.022.8923115
AACGCAG142900.022.7206136
TACGGGG34750.022.63396615
TTCTACG903.9945655E-521.38971116
CCCACGA650.003242262421.1510819
AGTGGTA4500.020.7706682
GTACGGG91850.020.749314
Produced by FastQC (version 0.11.5)