FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005414132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length91
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCCACAAGTCATACCCTGGCTTTTCAAAGACAGTGGGATGTCTCTT12.272727272727273No Hit
TATTTATCCACTAAGTAAAATGTTAGACTGAAATGATTCTTTGACAAGCT12.272727272727273No Hit
AAAAAAAGAAGATCATTTAAATTTTAAAATTATTATATAAATAAATTTTC12.272727272727273No Hit
ACTAAAAAACTTAAAACATAAATAGCAAAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
AACATGTACACCTACAGAAAGTAAAAAAAGAAGATACCCAATTGCAAGTT12.272727272727273No Hit
ATCAACGCAGAGTACGGGAGCAATGGAACAATATTGTGATTGCCTATGAG12.272727272727273No Hit
ACGAAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTAATATAAGGGG12.272727272727273No Hit
GTCCCGTACACGAAAGGGCACATATAATGATGACGAGTAGGGCGAGGCAC12.272727272727273No Hit
GTACGGGGAAATAAAAAAGAAAAAATAAAAAGGGACATCACAAGAAATAA12.272727272727273No Hit
ACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.272727272727273No Hit
ATGATACAATTATCCTCATTATAGATGATGCACGGGCCCCTGCGCTGGAT12.272727272727273No Hit
CTCATATACTCACCAATGTTGTTCCTAAAATGTTGCTGAGAGGCATAACT12.272727272727273No Hit
CCACACCCACTCTTCCCCACAACTACAAAACAACTTAAAACACCCTCACT12.272727272727273No Hit
TTCCGTAGTCAAGAGGAAAACAATCCAGACAGACAGATAACGCCCCCAAA12.272727272727273No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GATGAAAATACTGAAACGATTACTTTACCTGAAAATGTCCTGACTCAAAT12.272727272727273No Hit
GGATAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGAGCGGAGT12.272727272727273No Hit
GATACCACCCCGTACTCTGGTATCAACGCAGAGTACGGGGTCGTATCAAC12.272727272727273No Hit
ACGCAGAGTCGAGTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTATGTGT12.272727272727273No Hit
GTCGAATACCCACCGCCCCACACAACAATAACGAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GTCTAGGTGTCGTTCCTCAAGAAAGGTTTACAAAGAAAAAAAATTAAAAA12.272727272727273No Hit
GTCTAGGTAGTGTGCTAAATGAGGGGCAAGTTTTCGCTAACACCACAAGG12.272727272727273No Hit
TTTCTTTTGGTGGTTGGTCTTCGGGGTATTTTTTTTGGGTTTATCGTATA12.272727272727273No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAGTGTAGTG12.272727272727273No Hit
CTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATAAG12.272727272727273No Hit
GAGGTATAAAAACAGACTAAAAAAAACAAAAACTCATGAAATATAAAAAA12.272727272727273No Hit
ACGCAGAGTCGAGTAATTTTTTTTTTTTTTTTATTTTTTTTTTTGATGTG12.272727272727273No Hit
GCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTTAGTAGGGAAGA12.272727272727273No Hit
GGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCC12.272727272727273No Hit
AAATTGTGCATTACAATAATTAAAATCATTTGAACAAAAAAAAAAATAGC12.272727272727273No Hit
GGTATCAACGCAGAGTAAGGGATATTAAGCATAGAGCAAATGCTTTAAAC12.272727272727273No Hit
ACTGAAGAAGACAGAGACGCAAGAGAAAAATCCACTGCCTTCCAAAGAAA12.272727272727273No Hit
AAGCTGTTTAACTTTGTAAGATGCAAAGAGGTTGGATCAAGTTTAAATGA12.272727272727273No Hit
AAGTGCCAGCGTACAAATGGCTTTGATATGAGATGTATCTACGCCACACC12.272727272727273No Hit
ATGGCATTATGTAAAAATACAGCAGACGTTTTTCCTTTATTAGACTAACT12.272727272727273No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG12.272727272727273No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12.272727272727273No Hit
CAGTACTGTGTGTTAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAG12.272727272727273No Hit
GTGATTTTTCTGGGCCAGGTTGGGGACCTGTAATTGATGCTGTGCGTCAG12.272727272727273No Hit
GTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGAC12.272727272727273No Hit
CACTACTAGAAAAAAAAAAAAAAAAAAAAAAAAATCAGTTGCGGACTGTG12.272727272727273No Hit
AAACAAACCTTACAAATCCAATACATAACAAATAATAAAAAAAAAAAAAA12.272727272727273No Hit
GAATCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTA12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)