FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005414133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length61
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAACGACGCTGAGTCGGGTGTTTATGTTGTTAAGAAGTTTGTTTATAGTTGGTGGGAGT12.272727272727273No Hit
AGGGTGGGTGGTTACGGCTGGGGAATGGGAGCGTGGGGCTTTCTTTTGTTGTTTTTGTTGT12.272727272727273No Hit
GAATATATTATATTTTAATTTATATTATTTTAATCAAACAGAGTGGAATGGAAGGCAATGG12.272727272727273No Hit
ATATACTATATGTCATAAAAATTAATTAGATAGATTTGGTATTCAGAACAAAGGGCCTCCA12.272727272727273No Hit
GAGTACGGGCGAGATTTAGAAAGAATATATCAAACTCCACTCATTGGGCCAGACACCCTTG12.272727272727273No Hit
ATGCAGAGCTGGCGCGGCCGCCGGCGGGGAGAGTGTTGGTGTGGGTGGGCGCCGTGTCAGT12.272727272727273No Hit
CACCCCACAATAGGCTTAAAAACATATTCAATTCACGAACGTCTAAACAAAACCAATATCA12.272727272727273No Hit
ATACAAATTCTGCAAGCATGCTATAAAATAAACAAATCCATAAACACTTACAAATTAACCA12.272727272727273No Hit
GGGCGGGGGGGCGTGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG12.272727272727273No Hit
AACCACAGTACACGTTTTTTTTTAATATTAATATTTTTTTTAACCAAAGGCTAGTAGAAAT12.272727272727273No Hit
GGGGGAGGGGGGCGTACGGGGGGCGCCAATTATGGAGGGGTTCGAGGTCGGCGCGGGGGGG12.272727272727273No Hit
ACCCACCAGAATGACACATTTTCTAGACCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GTGGTATCAACGCAGAGTAGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTGT12.272727272727273No Hit
ATGTTTGTTTTATGTTTTTTTGTTTTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTT12.272727272727273No Hit
ACACAAAACACACACACAAACAAAAAAAACAATCAAATTCAAAAAACCCAAACACAACAAA12.272727272727273No Hit
ATCACCGCATAGTAAAGTTATAAATATATTTTTTTTTATTATTATTCTTTAAACACCCCCG12.272727272727273No Hit
GGCCCAAGGTGTCCTGCAGGCTGTAATGAAGTTTAATAAGAGTGCCATTTTTTTTTTTTTG12.272727272727273No Hit
GTCAAAAAGCAGAAACAACAAAAATGAAATAAAAAATTTTAATTTATGTCAGCATTTGATA12.272727272727273No Hit
CAATAATGAGAAAGATATAAAAGCTAATATCATCGTATAAATACAAGACACAACTATAAAC12.272727272727273No Hit
GTGGATGGGGTGGCAGTCAGAGGCGATAAAAGACGTAATAGCCTGCGATAAGGATCAGGGA12.272727272727273No Hit
CAATTAAGAAATAGATCAAAAAAAATAAATAATAACATGAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
CTAAAAACAGTCGCGCCCATCGTTCTCAGAAGTTCAAAGAACAGAAAGCGACAACAAACTA12.272727272727273No Hit
GGGGGGGGGGGGGGGTGGGGGGGTGGGGTGGGGCGGTGGGGGGGGGGGTGGGGTGGGGGGG12.272727272727273No Hit
AGATTCGGCCGTAATTTGAGTTAAACCTTCGCGAAAATTTGCATGCATTGCCTGAGCATCT12.272727272727273No Hit
ATCAAATACTAAAACACCAAAAAAAATACCCCGAAGACCAAACACAAAAAGAAACTTACTA12.272727272727273No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGTTTTAATTTAAAAAAA12.272727272727273No Hit
TTTCTGAATGGGGAAACCCACTTACAATAATTTATGTATCTCATGATGAATACATAGTCAT12.272727272727273No Hit
CACAAAGAATTATAAAAGACATCGGATGACTGGAGTAAATACTGAAACTTATCAACTTATG12.272727272727273No Hit
TACCAGCACTGGTCATGTCTAAAGGTCATCGTATTCAGGAAATTCCTGAACTTCATTTGCT12.272727272727273No Hit
GGTATCCACGCAGAGTCGAAGTTTTAATTGTTTTTTTATAATTTTTTTTTTTTTAAGAGCA12.272727272727273No Hit
CAAAAAAAACCAACCAAAGAAAAATATAATTAAAAAATGAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GCGCGCGACTGGCATTGTCATGGGGTGTGGAGAGGGGGTCGGGGGGGCGGTGGGGGTCTGC12.272727272727273No Hit
TACTCACACACTCAAAGGATGAACAAACTTCAAAAATAACATATTTTTCTTTGACAAAATC12.272727272727273No Hit
GTGCCTGGTAGAAGTGTTTGGGTTGCGTATTGTGAGAGTGGGTTGGGTGATGTTCGGTTGT12.272727272727273No Hit
GGCTCAGTGTTGTTTGGGATGTTTTCTTGTGTGTCTGGTTTTGGGTCTGCTCTGGTTGCGG12.272727272727273No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGTTTTTTTTG12.272727272727273No Hit
TGCTTATCCTCTTCAATCGTATCTTTGGAAGGAAGTGGATTTTTATCTTTATTATCTGTCA12.272727272727273No Hit
CCTCCAATAATCAACAACACCACAACCAACACCTTACAAAAACAACAAAACAACAACACAA12.272727272727273No Hit
GTCGTATCAACGCAGAGTGGAGAATATTTTTTTTTTATTTTTTTAATTAAAAGATAAAAGA12.272727272727273No Hit
CAACTGAAAAATCCTCCATATCAAAACACAAGCTATCGCTCCTCACCGTATCAAAAAAAAA12.272727272727273No Hit
AGGTAACTGATTAATATCTCACAGTGGACTGGAAATCGCTGAATGTTCTCTGCAAGTTTTA12.272727272727273No Hit
GTGGTGTCTCCGCAGCGTCGACGGTGTTTTGTTTTTTGTTGTTTTTTTTTTGGTGGTTAAG12.272727272727273No Hit
CAAAATAAAAAAGTGATCTATTATTTTTTTAATATCTTTAGGTATACATGAAAATTCTCAA12.272727272727273No Hit
GTTTCATTCATTATAACAAATTTCCAATAATCCTGTCAATTATATTTCTAAATTTTCCCCC12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)