FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559069
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT35920.6424967222292777No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.5500215536901528No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.37633995088262806No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC14700.2629371329835852No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.2525627427026002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8850.049.9458161
TGGTATC8850.049.941352
GTATCAA31400.040.336261
TATCAAC34950.036.3575132
CAACGCA36300.035.236375
TCAACGC36300.035.236374
ATCAACG36900.034.7785873
AACGCAG36900.034.6634256
CGATACG250.002789543733.99853524-25
GGTATCA16950.033.6003761
ACGCAGA41500.030.8184577
CGCAGAG42300.030.2356018
GCAGAGT44350.028.8380159
TACCGCG808.617059E-426.5566028
CAGAGTA24300.026.403610-11
GAGTACG27500.023.25392512-13
AGAGTAC23500.022.60136410-11
ACGGGAT4750.021.02352716-17
GTACGGG30600.020.89813614-15
GTATAGT1857.398121E-720.6766321
Produced by FastQC (version 0.11.5)