FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559069
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72451.2959044411333842No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT150.00578016355.00456233
TGGTATC6500.030.881652
GTGGTAT7150.028.8562971
GTATCAA24600.026.8387011
GGTATCA12700.025.3435541
ACGGGTA550.001224532825.00431416
AGAGTAC24850.024.45001611
AACGCAG27550.023.8436646
ACGCGAG701.7979986E-423.57338530
CAGAGTA25850.023.5020710
TCAACGC27750.023.4716284
TATCAAC28400.023.430852
CAACGCA28100.023.3748845
CGCAGAG28000.023.1680558
ACGCAGA28300.023.1146457
ATCAACG28350.023.0718153
CGCGCTA600.002034305822.91651728
AGTACGG27500.021.99985713
GAGTACG28050.021.76456512
GCAGAGT30350.021.3760649
Produced by FastQC (version 0.11.5)