FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560214
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8060.14387359116337683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA202.6173552E-454.97919530
GCGACCG257.843473E-444.01086412
TGGTATC11300.042.3140032
GTGGTAT12200.039.8753281
TTAGCGC400.00786026527.5067911
TTGAGCG400.00786369327.50433237
GTATCAA39950.025.7991451
AGAGTAC24800.024.51209811
GGTATCA20450.024.321963
ACGGGTC701.7960083E-423.57724816
CAGAGTA25900.023.57724810
TATCAAC44250.023.1016482
CCGTTAC600.002032370322.92027936
ATGATCG600.002036597322.912099
TACGGGT2500.022.00543215
ATCAACG47200.021.7160153
TCAACGC47800.021.328454
CAACGCA48050.021.274675
GCGTAAT650.00323641921.15528941
AACGCAG49400.020.6394926
Produced by FastQC (version 0.11.5)