FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025224
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58580.5713873260867869No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7700.036.0673682
GTGGTAT7800.035.980311
GTATCAA34000.027.1097321
GGTATCA17450.026.9624881
TATCAAC38000.024.3130512
AGAGTAC35700.024.0285611
TCAACGC39400.023.4434174
CAGAGTA37150.023.38559710
CAACGCA39500.023.3840665
ATCAACG39800.023.3448073
AACGCAG40800.022.7759656
ACGCAGA43250.021.6128987
CGGTCGA650.003242548621.1504329
CGCAGAG44000.020.9310578
AGTACGG40500.020.77847713
GCAGAGT45450.020.445759
GAGTACG42800.019.78942112
CTGCCGA7750.019.51964455
TACGGGC6400.018.9078115
GTACGGG44650.018.60085114
Produced by FastQC (version 0.11.5)