FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580402
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.3556155905734301No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT16020.27601558919507513No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14970.25792467979090355No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.15954459150726566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6150.057.3537142
GTGGTAT6700.052.659181
GGTATCA14150.039.954531
GTATCAA27350.039.1664351
TATCAAC29500.036.014432
CAACGCA32050.033.2816055
ATCAACG32050.033.2816053
TCAACGC32050.033.2816054
AACGCAG33050.032.146016
ACGCAGA34750.030.5707687
CGCAGAG35050.030.4303428
GCAGAGT36200.029.463639
AGCGTCA1203.9863153E-728.3313523
ACCGTCT600.00680803528.3289138
CAGAGTA25050.025.78439710-11
ACTGTCG1001.0561581E-425.496028
GTATATA1251.7462642E-523.8044871
GAGTACG28500.022.66410412-13
TCTATTC2104.5747583E-922.260353
ACGGGAT5150.021.45481116-17
Produced by FastQC (version 0.11.5)