FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580402
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33860.5833887546907144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4850.038.5417182
GTGGTAT5650.034.564951
GTATCAA26900.032.1072651
TATCAAC30950.028.4219072
GGTATCA11750.027.6229531
AGAGTAC30300.027.0473511
TCAACGC32350.026.500684
ATCAACG32600.026.3817423
CAGAGTA31250.026.31084610
AACGCAG33350.026.1247676
CAACGCA33050.026.1079275
ACGCAGA34200.025.3951057
CGCAGAG34450.025.0512528
CGGGATT1900.024.60630617
CGGGATA1700.024.26573217
GCAGAGT35850.024.1600369
CTAGTAC801.6166763E-524.0427863
AGTACGG33650.023.94603313
TGCCGAC3850.023.57852655
ACTATAC1052.1114283E-723.5521163
Produced by FastQC (version 0.11.5)