FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296673
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.5470669727275486No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10130.3414533847030232No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.33673438432213243No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.19078244397029726No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA4480.15100801218850382No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA4310.14527779744027935No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC4260.1435924401613898No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG3910.13179493920916294No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT3610.12168279553582564No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT3540.11932329534538028No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC3390.11426722350871162No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA3320.11190772331826625No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG3220.10853700876048712No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG3000.1011214367333731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT200.003217657863.7673231
TGGTATC4750.055.470062
GTGGTAT5250.051.013861
GCTAGCC250.00779260250.9966623
GTATCAA17400.040.312681
TATCAAC19250.036.426192
GGTATCA8900.034.391371
TCAACGC20500.034.205084
CAACGCA20650.034.1624155
ATCAACG20650.033.9566153
AACGCAG21250.033.1978266
GCTTGTA1101.8498031E-730.917491
CGCAGAG23950.029.4552758
ACGCAGA24000.029.393917
TTCGTCA300.006805144328.33147884-85
GCAGAGT25000.028.2181539
CACTGCG957.434926E-526.840359
TCTATAC808.599615E-426.560763
CAGAGTA14750.026.21862410-11
GACAGGC1159.125219E-625.8765951
Produced by FastQC (version 0.11.5)