FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680436
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.2850450716361706No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42670.25392219638236746No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.17608525406501646No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.13924957570535265No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA21890.1302638124867594No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT18550.1103880183476193No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.10997145978781696No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC18180.10818620881723554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28050.050.3022042
GTGGTAT29300.047.5930021
GTATCAA101000.038.7681581
TATCAAC112750.035.2433242
GGTATCA53450.034.9183461
TCAACGC119700.033.2325334
ATCAACG120400.033.039323
AACGCAG120550.032.962966
CAACGCA122000.032.6756945
ACGCAGA135400.029.3477467
CGCAGAG136350.029.0809298
GCAGAGT143650.027.75359
GTCGAGG14800.027.5671148
CAGAGTA79600.025.39010210-11
TCGAGGT16950.023.0695279
GACAGGT8250.022.674391
GAGTACG91000.021.90609412-13
AGAGTAC75650.021.85584810-11
AGTCGAG24250.020.1543087
AGCGTCA3800.020.1312473
Produced by FastQC (version 0.11.5)