FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454248
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26820.1844252149564586No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.17266656031158373No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.11676137770174001No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.10032676682381547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14750.049.8465082
GTGGTAT17150.043.629331
GTATCAA54750.039.8348581
TATCAAC60750.035.8882062
GGTATCA28600.034.040781
TCAACGC64750.033.802454
ATCAACG65050.033.5812233
GTCGAGG9750.033.1287168
CAACGCA66450.033.0655865
AACGCAG68900.031.8898166
CGCAGAG76350.028.7790938
ACGCAGA76950.028.6089447
TCGAGGT11350.027.3361639
GCAGAGT86350.025.5455679
AGTCGAG14750.024.779197
CAGAGTA51150.022.18623510-11
AGAGTAC47300.020.9369210-11
GAGTACG57950.019.47316612-13
GTGTAAG5550.019.1503641
GTACGGG61250.018.28489714-15
Produced by FastQC (version 0.11.5)