FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454248
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26210.18023060715916403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18700.039.6894232
GTGGTAT19350.038.9391561
GTATCAA65550.025.2546141
CCGATCG852.5695972E-522.64857546
TATCAAC72350.022.6443752
GGTATCA34750.022.5531371
AGAGTAC52400.021.10019311
ATCAACG78450.020.6726783
TCAACGC78650.020.5851614
CAGAGTA54800.020.52742210
CAACGCA80250.020.2432465
AACGCAG81200.019.9711846
CGATCGT909.806145E-418.33645247
AGTACGG59550.018.28963713
ACGCAGA91200.017.7825837
CCTAGCG1252.4821455E-517.60117717
CGCAGAG91650.017.5795358
TCTATAC2202.0918378E-1017.4921443
GAGTACG63750.017.214112
TACGGGG25100.017.09395415
Produced by FastQC (version 0.11.5)