FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588778
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.29840544116295664No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46620.29343306616783466No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.17881667545748997No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA26140.16452896502846842No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.15905305838827072No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC23440.14753477200716525No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA19930.12544232107947115No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA19840.12487584797876104No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC19700.12399466759987865No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT19080.12009229735054237No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC18780.11820405368150869No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT18580.11694522456881956No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18250.11486815653288251No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.11310579577511774No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT17200.1082593036912646No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG17170.10807047932436123No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA16620.10460869926446614No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC16230.10215398249472235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC33150.042.1772542
GTGGTAT34600.040.2982981
GTATCAA117450.037.8226741
AACGCAG133800.033.318496
TATCAAC139300.032.186022
TCAACGC139100.032.079544
ATCAACG140800.031.6922153
CAACGCA141900.031.5363855
GGTATCA66850.030.713881
ACAGGTA6900.030.1881121
ACGCAGA148050.030.0541327
CGCAGAG149200.029.8794528
GTCGAGG14600.028.8169678
GACAGGC2550.028.339861
GCAGAGT161150.027.6918669
GACAGGT5600.026.5686191
GCTTGTA9050.025.834681
GGCACCG9950.024.7741189
GCTTGGG3700.024.1271781
CAGAGTA99550.023.99396310-11
Produced by FastQC (version 0.11.5)