FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588778
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29190.18372610899697756No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA25240.15886423402136735No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC20870.13135881790911No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTT19720.12412055051114756No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGG19250.12116230209632813No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT17510.11021048881593276No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC17410.1095810742595882No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT17410.1095810742595882No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT16360.10297222141797029No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG16290.1025316312285291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC36800.033.5429082
GTGGTAT37600.032.988961
GTATCAA131400.026.037841
AGAGTAC99900.024.14198511
CAGAGTA100950.024.13605710
GACAGGT4450.023.48571
GCACCGC6950.023.34565710
TTGTACG1800.022.909063
AGCCGTC852.566459E-522.65237649
TATCAAC150300.022.6079752
AACGCAG150400.022.4256086
ATCAACG153700.022.0356243
TCAACGC154050.021.931334
GCTTGTA7650.021.930491
CCCAGTG7700.021.78396820
CAACGCA157400.021.499495
CTGCCCA7650.021.20945417
GGCCGCG7550.021.12812440
AACGGCC7550.021.12812437
GACACAT7700.021.07239326
Produced by FastQC (version 0.11.5)