FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862511
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.3049236473505845No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.2735037582129387No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.18121508015549948No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.16162112715084215No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.12023035068538257No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.11072322555886244No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8720.10110015988201891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17250.044.592532
GTGGTAT17100.043.5076561
GTATCAA57800.038.0265051
TATCAAC65650.034.179982
GGTATCA31950.033.6645931
TCAACGC69700.032.2548874
AACGCAG70300.031.8586946
ATCAACG70750.031.7761963
CAACGCA71750.031.3925515
ATACGGG1251.6652848E-830.6094991
GGTACGG905.15497E-528.332273
CGCAGAG79800.028.1741358
ACGCAGA80050.027.979967
GCTTGTA3850.026.501731
GCAGAGT85450.026.3625139
GTACGGC1051.4705845E-424.2848033
GTCGAGG8700.023.4487578
CAGAGTA50350.023.34095610-11
AAGTACG1903.862442E-822.3753661
AGCGTCA1553.7079917E-621.934663
Produced by FastQC (version 0.11.5)