FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325525
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.29214346056370477No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.2319330312571999No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.14591813224790723No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA4570.14038860302588127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.044.1407431
TGGTATC3300.041.2060132
GGTATCA6200.039.0667881
TATCAAC15300.038.3276522
TCAACGC15500.037.2847944
CAACGCA15800.036.576865
ATCAACG15900.036.3468133
TATACTA601.5944103E-435.411425
AACGCAG16450.035.1315736
GTGGTAT3950.034.4252781
CTAGGTG500.002788089233.994965
CGCAGAG17600.032.836048
ACGCAGA17650.032.7430237
CTATCGG652.551574E-432.687466
GCAGAGT18200.031.7535369
ACTATCG703.9405125E-430.3526445
TATCGGT853.486765E-529.9955567
ATCGGTC808.6055783E-426.5585638
CAGAGTA13000.025.98653410-11
AGAGTAC12600.023.43898610-11
Produced by FastQC (version 0.11.5)