FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719772
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.6025519192188638No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.467231289908471No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT28280.3929021968067665No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.29453771472077267No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.1257342602935374No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC7830.10878444840866275No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.10017060958192317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10400.059.2720571
TGGTATC11800.052.2216342
GTATCAA40350.038.5614051
TATCAAC43850.035.4712032
TCAACGC46650.033.342174
CAACGCA47800.032.5400055
ATCAACG47800.032.5400053
GGTATCA21950.032.5379451
AACGCAG49250.031.4956866
CGCAGAG54400.028.590158
ACGCAGA54350.028.5382657
GCAGAGT56650.027.5296339
CAGAGTA32150.025.64218510-11
ATACCGC1509.806172E-825.4985626
GAGTACG35600.022.91846512-13
AGAGTAC30400.022.2258210-11
GATACCG1955.1017196E-821.7936425
GTACGGG38350.021.05341114-15
GACAGGT3250.020.9291671
ACGGGAT6750.019.83083716-17
Produced by FastQC (version 0.11.5)