FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866604
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57520.6637403012217806No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.6471237150993995No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.3724884722433776No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.3487175226516379No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA27670.31929231805992125No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26120.30140640938652485No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC25710.2966752980600136No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.2845590373457773No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT23650.2729043484682738No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG23180.2674808793866633No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.23551702969291624No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT19670.2269779507133593No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA19660.22686255775417608No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA18840.21740033510115347No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC18410.21243843785627575No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA17830.20574564622365No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA17170.19812971091755865No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG16950.1955910658155282No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG16240.18739816571352083No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA16010.18474412765230716No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT15530.17920526561151345No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15400.1777051571421318No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT15300.17655122755029978No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT15170.17505111908091817No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT14870.1715893303054221No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC14560.1680121485707428No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC14490.16720439785646038No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT14350.16558889642789557No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA14350.16558889642789557No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA14190.16374260908096433No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG13910.16051160622383465No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC13840.15970385550955224No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13830.15958846255036901No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC12620.1456259144892015No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA12470.1438950201014535No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG12170.14043323132595742No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.13812537214229337No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG11600.13385583265251488No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA11500.13270190306068286No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT11470.13235572418313324No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.13200954530558365No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG11390.13143258050966763No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.11608531693830168No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG9770.11273892112198883No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT9570.11043106193832476No Hit
GTGGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA9520.10985409714240875No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG9390.10835398867302713No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.10604612948936307No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC9130.10535377173426386No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT9100.10500759285671427No Hit
GGGAATGAGAACGGAAAATCTCACTAAGGAAGACGAAGTCGATGGAGCAA8900.1026997336730502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC36600.050.7412642
GTGGTAT37500.048.069651
AAGTACG2750.046.3793751
GACAGGT3400.042.514431
GTATCAA119550.037.4468351
ATACGGG1950.034.8836331
TATCAAC136400.034.209672
TCCGAGG751.4839205E-533.997818
GTCCGAG751.4839205E-533.997817
ATATCAA6450.032.9569241
GCTTGTA3800.032.445221
AACGCAG149400.031.261376
GTCGAGG21900.031.0482258
ATCAACG150900.030.8942973
TCAACGC151950.030.7087784
CAACGCA156100.029.892375
TCGAGGT21850.029.7595259
GGTATCA65300.029.6764933
ACAGGTA4900.028.6321681
ACGCAGA171500.027.3568387
Produced by FastQC (version 0.11.5)